Modified Rose Bengal assay for surface hydrophobicity evaluation of cationic solid lipid nanoparticles (cSLN)
Surface hydrophobicity of nanocarriers influences protein binding and subsequently fate in blood circulation. Therefore, characterization of surface properties of nanocarriers provides important preclinical information. Rose Bengal adsorption method was developed as easy and quick method for surface hydrophobicity characterization and was successfully used for various types of nanocarriers. Here, we revised the method for the needs of characterization of cationic solid lipid nanoparticles (cSLN). Overall, we confirmed that Rose Bengal adsorption can provide useful information about (cationic) SLN, however, performing and evaluation of the assay need special attention in order to obtain the desired results.
https://doi.org/10.1016/j.ejps.2011.12.016
20 Years of Lipid Nanoparticles (SLN & NLC): Present State of Development & Industrial Applications
The scientific community considers the lipid nanoparticles as very promising delivery system, as shown by the many research groups working meanwhile with SLN and NLC. Lipid nanoparticles already made it to the cosmetic market. This proves the makeability of products and of large scale production ability – both being important prerequisites for a pharmaceutical product. Various companies are working with these nanoparticles, and this decade will now show if the lipid nanoparticles reach the final goal: pharmaceutical products.
https://doi.org/10.2174/157016311796799062
Nanosuspensions: a new approach for organ and cellular targeting in infectious diseases
The challenges in treating AIDS, malaria, tuberculosis, chagas and others can be reduced to certain limit by use of correct formulation type i.e., nanosuspensions. These diseases demand attention of pharma R & D’s to invest resources for developing effective drug delivery systems. Many published reports confirmed that the nanocrystal would serve as an ideal industrially feasible option and can save several active compounds coming from high throughput screening. Intracellular drug targeting at therapeutic level is very essential for these pathogens which form latent sanctuaries at cellular and subcellular level.
https://doi.org/10.1007/s40005-013-0051-x
Stavudine entrapped lipid nanoparticles for targeting lymphatic HIV reservoirs
The development and characterization of lipid nanoparticle of stavudine with robust particle size control, stability and superior targeting of antiretroviral drug molecules is reported. The blood clearance, lymph kinetic, gamma imaging and biodistribution for radiolabeled pure drug, blank and drug loaded nanoparticles were carried out in rats. The prepared lipid nanoparticles showed enhanced levels in major HIV reservoir sites in body. Macrophage uptake study revealed the potential of prepared lipid nanoparticles for targeting intracellular compartments and eradication of viral load from the cellular and anatomical reservoir sites focusing on the
https://pubmed.ncbi.nlm.nih.gov/21612153/
Nanocrystals: industrially feasible multifunctional formulation technology for poorly soluble actives
Poor solubility of new drugs and their related low oral bioavailability and general delivery problems are becoming a major challenge. Nanocrystals being a kind of “universal” formulation approach for these molecules are reviewed in this paper regarding the industrial feasibility, i.e., industrially available production processes (bottom-up and top-down technologies), regulatory aspects and nanotoxicology. This article also includes second generation nanocrystals (<< 100 nm) as smartCrystals. The status of products on the market and in clinical phases is presented. The different special features of nanocrystals, which are exploited in different products are described (tablets, capsule, aqueous nanosuspension).
https://doi.org/10.1016/j.ijpharm.2010.07.044
Nano-crystalline Suspensions by Top Down Processes: Review of Manufacturing Considerations
This article successfully reviews practical considerations to manufacture nano-crystalline suspensions by top-down processes. The factors like formulation Composition, selection of milling steps and application of engineering principles are key aspects. In order to make a successful nano-crystalline suspension as a drug delivery system, the formulation and process engineering, have to be addressed based on physico-chemical principles, engineering methodologies and compliance to pharmaceutical regulation. Theoretical considerations of industrially employed milling technics like SBM and HPH are reviewed. The key fact on mathematical modelling and prediction tools (simulation scaling-down and scaling-up) are highlighted in detail. Formulation scientists and engineers needs to evaluate these factors and adjust according to API physicochemical properties and needs to be optimized case to case basis.
https://doi.org/10.2174/2210303108666180706144005